Nucleic acid mutation analysis using catalytic DNA

Nucleic acid-triggered catalytic drug release.

We propose a concept for the rational design and synthesis of highly selective chemotherapeutic agents that makes direct use of genetic information about the disease state. The key idea is to use the mRNA or DNA specific to the disease state to trigger the catalytic release of a cytotoxic drug by promoting the association of a prodrug with a catalyst capable of releasing the drug. We demonstrat...

Structural Biochemistry/Nucleic Acid/DNA/DNA structure

Deoxyribonucleic acid (DNA) stores information for the synthesis of specific proteins. DNA has deoxyribose as its sugar. DNA consists of a phosphate group, a sugar, and a nitrogenous base. The structure of DNA is a helical, double-stranded macromolecule with bases projecting into the interior of the molecule. These two strands are always complementary in sequence. One strand serves as a templat...

Antibody-powered nucleic acid release using a DNA-based nanomachine

A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we report the rational design of a modular DNA-based nanomachine that can reversibly load and release a molecular cargo on binding to ...

Nucleic Acid Interaction Analysis

The concept of double stranded DNA forming triple helical complexes with a third strand of nucleic acids via so called Hoogsteenhydrogen bonds has been well established for the last 50 years. However, the topic reentered the big stage with recent publications pointing to an existence of these structures in vivo, highlighting their putative role in chromatin organization and transcriptional regu...

nucleic acid testing for the detection of hbv dna